NEST Vacuum Filtration System is mainly applied in the isolation and purification of large volume samples in tissue culture medium and biological fluid. The system consists of a membrane diaphragm, a receiver bottle connected to a transparent graduated polystyrene bottle top vacuum filter by a connector. With the pressure differential conducted by the vacuum pump, the Vacuum Filtration System can filtrate the tissue culture medium and other lab fluid solution in large volumes even up to several Liters. The filtrated samples can be directly gathered in aseptic storage bottles.